Hotspot

psbZ-trnS-UGA

LSC · 28167-28527 · 361 bp

Back to Vigna

Pi 0.013
MarkerSeek score 57.80
Primer available yes
Label psbZ-trnS-UGA

Feature Metadata

label_name
psbZ-trnS-UGA
feature_type
igs
region
LSC
length_bp
361
variable_sites
7
pi
0.013
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnS-UGA_p1 TTGACCAACCTTCAGGAGAA CAACCACTCAGCCATCTCTC 45.0 55.0 56.3 58.0 484-485 bp 1.0 67.8
psbZ-trnS-UGA_p2 TTTGACCAACCTTCAGGAGA CAACCACTCAGCCATCTCTC 45.0 55.0 56.3 58.0 485-486 bp 1.0 67.8
psbZ-trnS-UGA_p3 TTGACCAACCTTCAGGAGAA GTTTTCAAGACCGGGGCTAT 45.0 50.0 56.3 57.9 504-505 bp 1.0 66.9
psbZ-trnS-UGA_p4 TTTGACCAACCTTCAGGAGA GTTTTCAAGACCGGGGCTAT 45.0 50.0 56.3 57.9 505-506 bp 1.0 66.9
psbZ-trnS-UGA_p5 TTTGACCAACCTTCAGGAGAA CAACCACTCAGCCATCTCTC 42.9 55.0 57.1 58.0 485-486 bp 1.0 65.9