Hotspot

trnG-UCC-psbZ

LSC · 27301-27977 · 677 bp

Back to Vigna

Pi 0.0187
MarkerSeek score 66.40
Primer available yes
Label trnG-UCC-psbZ

Feature Metadata

label_name
trnG-UCC-psbZ
feature_type
igs
region
LSC
length_bp
677
variable_sites
19
pi
0.0187
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-psbZ_p1 CGTCTTCTCCTTGGCAAAGA CTTCTCCTGAAGGTTGGTCA 50.0 50.0 57.8 56.8 818-832 bp 1.0 71.9
trnG-UCC-psbZ_p2 CGTCTTCTCCTTGGCAAAGA CTTCTCCTGAAGGTTGGTCAA 50.0 47.6 57.8 57.5 818-832 bp 1.0 69.8
trnG-UCC-psbZ_p3 CGTCTTCTCCTTGGCAAAGA TCTCCTGAAGGTTGGTCAAA 50.0 45.0 57.8 56.3 816-830 bp 1.0 68.0
trnG-UCC-psbZ_p4 CGTCTTCTCCTTGGCAAAGA TTCTCCTGAAGGTTGGTCAA 50.0 45.0 57.8 56.3 817-831 bp 1.0 68.0
trnG-UCC-psbZ_p5 CGTCTTCTCCTTGGCAAAGA TTCTCCTGAAGGTTGGTCAAA 50.0 42.9 57.8 57.1 817-831 bp 1.0 66.2