Hotspot

rps16

LSC · 4660-5710 · 1051 bp

Back to Vietsenia

Pi 0.0202

MarkerSeek score 57.40

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1051
variable_sites: 21
pi: 0.0202
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	CCTTTCAGGATCAGTCGTGG	CATACCAGTTAAACCCCGGG	55.0	55.0	58.0	58.0	1638 bp	0.5	59.5
rps16_p2	TCAGGATCAGTCGTGGTCTT	CATACCAGTTAAACCCCGGG	50.0	55.0	58.1	58.0	1634 bp	0.5	59.0
rps16_p3	TTCAGGATCAGTCGTGGTCT	CATACCAGTTAAACCCCGGG	50.0	55.0	58.1	58.0	1635 bp	0.5	59.0
rps16_p4	AAAGCCGAGTACTCTACCGT	CATACCAGTTAAACCCCGGG	50.0	55.0	58.2	58.0	1543 bp	0.5	58.3
rps16_p5	AAGCCGAGTACTCTACCGTT	CATACCAGTTAAACCCCGGG	50.0	55.0	58.2	58.0	1542 bp	0.5	58.3