Hotspot

ndhG-ndhI

SSC · 121158-121570 · 413 bp

Back to Vietsenia

Pi 0.0291

MarkerSeek score 57.10

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 413
variable_sites: 12
pi: 0.0291
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CCCATTCCCAACAGTCCAAA	TGGAAACCGGTATGCGAAAA	50.0	45.0	58.0	58.1	967-989 bp	1.0	80.6
ndhG-ndhI_p2	CCCATTCCCAACAGTCCAAA	TTGGAAACCGGTATGCGAAA	50.0	45.0	58.0	58.1	968-990 bp	1.0	80.6
ndhG-ndhI_p3	CCCATTCCCAACAGTCCAAA	ATTGGAAACCGGTATGCGAA	50.0	45.0	58.0	57.9	969-991 bp	1.0	80.6
ndhG-ndhI_p4	CCCATTCCCAACAGTCCAAA	AATTGGAAACCGGTATGCGA	50.0	45.0	58.0	57.9	970-992 bp	1.0	80.5
ndhG-ndhI_p5	TCCGTACCAAGACGTATCGA	TGGAAACCGGTATGCGAAAA	50.0	45.0	58.0	58.1	1039-1061 bp	1.0	80.4