Hotspot

ccsA-ndhD

SSC · 117612-117961 · 350 bp

Back to Vietsenia

Pi 0.0253

MarkerSeek score 61.90

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 350
variable_sites: 8
pi: 0.0253
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGTGGCTTCTATGGGCTTTC	TCAGTTGAGAAGGTCGAAGC	50.0	50.0	57.9	57.6	475-507 bp	1.0	80.7
ccsA-ndhD_p2	TGTGGCTTCTATGGGCTTTC	CAGTTGAGAAGGTCGAAGCT	50.0	50.0	57.9	57.6	474-506 bp	1.0	80.7
ccsA-ndhD_p3	GATTGTGGCTTCTATGGGCT	TCAGTTGAGAAGGTCGAAGC	50.0	50.0	57.6	57.6	478-510 bp	1.0	78.9
ccsA-ndhD_p4	GATTGTGGCTTCTATGGGCT	CAGTTGAGAAGGTCGAAGCT	50.0	50.0	57.6	57.6	477-509 bp	1.0	78.9
ccsA-ndhD_p5	GTGGCTTCTATGGGCTTTCT	TCAGTTGAGAAGGTCGAAGC	50.0	50.0	57.6	57.6	474-506 bp	1.0	78.4