Hotspot

rbcL-accD

LSC · 57919-58523 · 605 bp

Back to Vietsenia

Pi 0.0287
MarkerSeek score 65.50
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
605
variable_sites
17
pi
0.0287
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 AGTTTGAATTCGAAGCAATGGA ACATCGAATTAAACCGCCGT 36.4 45.0 56.9 58.3 665 bp 1.0 56.4
rbcL-accD_p2 AAGTTTGAATTCGAAGCAATGGA ACATCGAATTAAACCGCCGT 34.8 45.0 57.5 58.3 666 bp 1.0 53.7
rbcL-accD_p3 CAAGTTTGAATTCGAAGCAATGG ACATCGAATTAAACCGCCGT 39.1 45.0 57.5 58.3 667 bp 1.0 53.3
rbcL-accD_p4 GTTTGAATTCGAAGCAATGGA ACATCGAATTAAACCGCCGT 38.1 45.0 55.4 58.3 664 bp 1.0 52.6
rbcL-accD_p5 AGTTTGAATTCGAAGCAATGG ACATCGAATTAAACCGCCGT 38.1 45.0 55.4 58.3 665 bp 1.0 52.5