Hotspot

trnD-GUC-trnY-GUA

LSC · 30831-31308 · 478 bp

Back to Vietsenia

Pi 0.0109

MarkerSeek score 66.70

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 478
variable_sites: 5
pi: 0.0109
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	TTTATTCCGTCGGGACTGAC	TGGCAATATGTCTACGCTGG	50.0	50.0	57.3	57.8	584-602 bp	1.0	76.3
trnD-GUC-trnY-GUA_p2	TTTATTCCGTCGGGACTGAC	GGCAATATGTCTACGCTGGT	50.0	50.0	57.3	57.8	583-601 bp	1.0	76.3
trnD-GUC-trnY-GUA_p3	TTTATTCCGTCGGGACTGAC	GGGGACGGACTGTAAATTCG	50.0	55.0	57.3	58.4	605-623 bp	1.0	75.3
trnD-GUC-trnY-GUA_p4	TTTATTCCGTCGGGACTGAC	GGGACGGACTGTAAATTCGT	50.0	50.0	57.3	57.6	604-622 bp	1.0	75.1
trnD-GUC-trnY-GUA_p5	TTTATTCCGTCGGGACTGAC	TAGAATGGCTTCTTGCTGGG	50.0	50.0	57.3	57.6	645-663 bp	1.0	74.5