Hotspot

psbK-psbI

LSC · 7297-7676 · 380 bp

Back to Vietsenia

Pi 0.016

MarkerSeek score 62.90

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 380
variable_sites: 6
pi: 0.016
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TTTTTATTCGTCACGCCCGG	50.0	50.0	57.9	59.2	518-524 bp	1.0	71.8
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	TTTTTATTCGTCACGCCCGG	45.0	50.0	58.3	59.2	515-521 bp	1.0	69.8
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	TTTTTATTCGTCACGCCCGG	45.0	50.0	58.3	59.2	516-522 bp	1.0	69.8
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	TTTTTATTCGTCACGCCCGG	45.0	50.0	58.3	59.2	517-523 bp	1.0	69.8
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	GGGATTACGTCCTGGATCAT	50.0	50.0	57.9	56.5	500-506 bp	1.0	69.3