Hotspot

trnK-UUU-rps16

LSC · 4230-4659 · 430 bp

Back to Vietsenia

Pi 0.0242
MarkerSeek score 66.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
430
variable_sites
10
pi
0.0242
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TTTTCTTGAAAAGGGTGCGC 50.0 45.0 58.2 57.8 542-558 bp 1.0 80.7
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT TTTTCTTGAAAAGGGTGCGC 50.0 45.0 58.2 57.8 541-557 bp 1.0 80.7
trnK-UUU-rps16_p3 CCGCACTTAAAAGCCGAGTA TTTTCTTGAAAAGGGTGCGC 50.0 45.0 58.3 57.8 551-567 bp 1.0 79.7
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TATTTTCAAAAGGGCGGGGG 50.0 50.0 58.2 58.4 499-515 bp 1.0 79.3
trnK-UUU-rps16_p5 AAAGCCGAGTACTCTACCGT TATTTTCAAAAGGGCGGGGG 50.0 50.0 58.2 58.4 500-516 bp 1.0 79.2