Hotspot

rpl2

IRa · 153863-155338 · 1476 bp

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Pi 0.358

MarkerSeek score 70.60

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: IRa
length_bp: 1476
variable_sites: 63
pi: 0.358
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.12
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	CGCTTCAACCGGGTTATTCT	AACGTGTCACAGCTTACTCC	50.0	50.0	58.3	57.8	1610 bp	0.5	56.7
rpl2_p2	ATCATTACGCTTCAACCGGG	AACGTGTCACAGCTTACTCC	50.0	50.0	58.3	57.8	1617 bp	0.5	56.1
rpl2_p3	ACGCTTCAACCGGGTTATTC	AACGTGTCACAGCTTACTCC	50.0	50.0	58.6	57.8	1611 bp	0.5	54.4
rpl2_p4	TACGCTTCAACCGGGTTATT	AACGTGTCACAGCTTACTCC	45.0	50.0	57.2	57.8	1612 bp	0.5	52.7
rpl2_p5	TTACGCTTCAACCGGGTTAT	AACGTGTCACAGCTTACTCC	45.0	50.0	57.2	57.8	1613 bp	0.5	52.7