Hotspot

rpl32-trnL-UAG

SSC · 115097-116017 · 921 bp

Back to Vicatia

Pi 0.0729
MarkerSeek score 80.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
921
variable_sites
63
pi
0.0729
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGTAAGGGATATTGGGCAGC AGCGTGTCTACCGATTTCAC 55.0 50.0 58.1 58.0 1025-1052 bp 1.0 88.4
rpl32-trnL-UAG_p2 GTAAGGGATATTGGGCAGCC AGCGTGTCTACCGATTTCAC 55.0 50.0 58.1 58.0 1024-1051 bp 1.0 88.4
rpl32-trnL-UAG_p3 GGTAAGGGATATTGGGCAGC CAGCGTGTCTACCGATTTCA 55.0 50.0 58.1 58.0 1026-1053 bp 1.0 88.4
rpl32-trnL-UAG_p4 GTAAGGGATATTGGGCAGCC CAGCGTGTCTACCGATTTCA 55.0 50.0 58.1 58.0 1025-1052 bp 1.0 88.4
rpl32-trnL-UAG_p5 GTAAGGGATATTGGGCAGCC CTCGCACTGCTTCCTAAGAG 55.0 55.0 58.1 58.1 1045-1072 bp 1.0 87.7