Hotspot

rbcL-accD

LSC · 57681-58301 · 621 bp

Back to Vicatia

Pi 0.0469

MarkerSeek score 78.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 621
variable_sites: 28
pi: 0.0469
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTGCTGCTTGTGAAGTATG	ATCGAACCACCATCTTTCCA	50.0	45.0	57.5	56.8	686-705 bp	1.0	72.3
rbcL-accD_p2	GCTGCTGCTTGTGAAGTATG	TCGAACCACCATCTTTCCAT	50.0	45.0	57.5	56.8	685-704 bp	1.0	72.3
rbcL-accD_p3	GCTGCTGCTTGTGAAGTATG	AATCGAACCACCATCTTTCCA	50.0	42.9	57.5	57.6	687-706 bp	1.0	70.2
rbcL-accD_p4	TGCTGCTTGTGAAGTATGGA	ATCGAACCACCATCTTTCCA	45.0	45.0	57.1	56.8	684-703 bp	1.0	69.8
rbcL-accD_p5	TGCTGCTTGTGAAGTATGGA	TCGAACCACCATCTTTCCAT	45.0	45.0	57.1	56.8	683-702 bp	1.0	69.8