Hotspot

psbA-trnK-UUU

LSC · 1315-1528 · 214 bp

Back to Vicatia

Pi 0.0909

MarkerSeek score 77.50

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 214
variable_sites: 18
pi: 0.0909
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	GCTTTCGCGTCTCTCTAAAA	CTAGTTCCGGGTTCGAATCC	45.0	55.0	56.2	57.8	265-275 bp	1.0	73.2
psbA-trnK-UUU_p2	TCGCGTCTCTCTAAAATAGCAG	CTAGTTCCGGGTTCGAATCC	45.5	55.0	58.0	57.8	261-271 bp	1.0	71.3
psbA-trnK-UUU_p3	TCGCGTCTCTCTAAAATAGCA	CTAGTTCCGGGTTCGAATCC	42.9	55.0	57.0	57.8	261-271 bp	1.0	71.3
psbA-trnK-UUU_p4	GCTTTCGCGTCTCTCTAAAA	ACTCCATCCGACTAGTTCCG	45.0	55.0	56.2	58.6	276-286 bp	1.0	69.9
psbA-trnK-UUU_p5	GCTTTCGCGTCTCTCTAAAAT	CTAGTTCCGGGTTCGAATCC	42.9	55.0	56.7	57.8	265-275 bp	1.0	69.4