Hotspot

trnH-GUG-psbA

LSC · 79-252 · 174 bp

Back to Vicatia

Pi 0.191

MarkerSeek score 79.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 174
variable_sites: 31
pi: 0.191
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	AGCTCCATCTACAAATGGGT	55.0	45.0	58.1	56.2	217-218 bp	1.0	76.5
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	AGCTCCATCTACAAATGGGT	50.0	45.0	57.9	56.2	231-232 bp	1.0	76.5
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	AGCTCCATCTACAAATGGGT	50.0	45.0	57.9	56.2	230-231 bp	1.0	76.5
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	AGCTCCATCTACAAATGGGT	50.0	45.0	57.9	56.2	232-233 bp	1.0	76.5
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	AAGCTCCATCTACAAATGGGT	55.0	42.9	58.1	57.0	218-219 bp	1.0	74.7