Hotspot

ycf1

SSC · 127719-133403 · 5685 bp

Back to Viburnum

Pi 0.00998

MarkerSeek score 43.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5685
variable_sites: 275
pi: 0.00998
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GACTGTTGTTCCAACTCGGT	GATGGCCTTGTCCACACTAG	50.0	55.0	58.1	58.0	675-699 bp	0.96	84.8
ycf1_p2	GACTGTTGTTCCAACTCGGT	ACGAAGGGGACTAAACAGGA	50.0	50.0	58.1	58.0	1163-1187 bp	0.96	83.1
ycf1_p3	CTGTTGTTCCAACTCGGTGA	ACGAAGGGGACTAAACAGGA	50.0	50.0	58.1	58.0	1161-1185 bp	0.96	83.1
ycf1_p4	TTGATGATCTACTGGCACGC	ACGAAGGGGACTAAACAGGA	50.0	50.0	58.1	58.0	1200-1224 bp	0.96	82.4
ycf1_p5	TGACTGTTGTTCCAACTCGG	ACGAAGGGGACTAAACAGGA	50.0	50.0	58.1	58.0	1164 bp	0.32	54.2