Hotspot

rps15-ycf1

SSC · 127314-127718 · 405 bp

Back to Viburnum

Pi 0.0248

MarkerSeek score 58.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 405
variable_sites: 47
pi: 0.0248
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CCGCAGACCTCTCTGAGATA	TGAGATGGGACGATGCTTTG	55.0	50.0	58.0	58.0	1263-1279 bp	1.0	85.5
rps15-ycf1_p2	AACACCATGCATTGATGGGT	TGAGATGGGACGATGCTTTG	45.0	50.0	58.1	58.0	1541-1557 bp	1.0	84.8
rps15-ycf1_p3	CTTGTAGCTGGTGCAGTCAT	TGAGATGGGACGATGCTTTG	50.0	50.0	57.9	58.0	1486-1502 bp	1.0	84.5
rps15-ycf1_p4	CCTAACTGCTCTGGTGCATT	TGAGATGGGACGATGCTTTG	50.0	50.0	57.9	58.0	1735-1751 bp	1.0	84.0
rps15-ycf1_p5	ATTGCCTAACTGCTCTGGTG	TGAGATGGGACGATGCTTTG	50.0	50.0	57.9	58.0	1739-1755 bp	1.0	83.9