Hotspot

psaC-ndhE

SSC · 121345-121608 · 264 bp

Back to Viburnum

Pi 0.0165

MarkerSeek score 54.80

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 264
variable_sites: 15
pi: 0.0165
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	CTTTACATCCGTCCCAAGGG	AAGCAGCTATTGGATTGGCT	55.0	45.0	58.0	57.6	439-462 bp	1.0	83.1
psaC-ndhE_p2	CAAGCTCGGACACATTGAGT	AAGCAGCTATTGGATTGGCT	50.0	45.0	58.2	57.6	395-418 bp	1.0	82.1
psaC-ndhE_p3	TCTAATACATCGGTGGGGCA	AAGCAGCTATTGGATTGGCT	50.0	45.0	58.2	57.6	413-436 bp	1.0	82.0
psaC-ndhE_p4	TTTACATCCGTCCCAAGGGA	AAGCAGCTATTGGATTGGCT	50.0	45.0	58.3	57.6	438-461 bp	1.0	80.7
psaC-ndhE_p5	CGGACACATTGAGTACACCC	AAGCAGCTATTGGATTGGCT	55.0	45.0	58.3	57.6	389-412 bp	0.96	79.2