Hotspot

ycf1-ndhF

SSC · 113587-113593 · 7 bp

Back to Viburnum

Pi 0.198

MarkerSeek score 60.80

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 7
variable_sites: 5
pi: 0.198
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	ACGAAGGGGACTAAACAGGA	GGTAAGACGATTGGCCGAAT	50.0	50.0	58.0	58.1	428-871 bp	0.92	85.2
ycf1-ndhF_p2	ACAGGAACAAGAGGGATCCA	GGTAAGACGATTGGCCGAAT	50.0	50.0	58.0	58.1	414-857 bp	0.92	85.1
ycf1-ndhF_p3	AAGAGGGATCCACCGAAGAA	GGTAAGACGATTGGCCGAAT	50.0	50.0	58.0	58.1	406-849 bp	0.92	85.1
ycf1-ndhF_p4	GCAGAATACCGTCACCCATT	GGTAAGACGATTGGCCGAAT	50.0	50.0	58.0	58.1	517-960 bp	0.92	84.7
ycf1-ndhF_p5	GATGGCCTTGTCCACACTAG	GGTAAGACGATTGGCCGAAT	55.0	50.0	58.0	58.1	327-383 bp	0.76	81.2