Hotspot

trnE-UUC-trnT-GGU

LSC · 32694-33504 · 811 bp

Back to Viburnum

Pi 0.0157
MarkerSeek score 68.70
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
811
variable_sites
70
pi
0.0157
unique_haplotype_count
19
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CACGATCAACTCCCTTTCGT GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 938-1013 bp 1.0 89.1
trnE-UUC-trnT-GGU_p2 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 924-999 bp 1.0 88.5
trnE-UUC-trnT-GGU_p3 CACGATCAACTCCCTTTCGT CCGATGACTTACGCCTTACC 50.0 55.0 57.9 58.2 935-1010 bp 1.0 87.9
trnE-UUC-trnT-GGU_p4 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 865-940 bp 1.0 87.9
trnE-UUC-trnT-GGU_p5 TTTCGTAGTACCCTACCCCC CCGATGACTTACGCCTTACC 55.0 55.0 57.8 58.2 921-996 bp 1.0 87.4