Hotspot

petD

LSC · 80641-81896 · 1256 bp

Back to Vesalea

Pi 0.0101

MarkerSeek score 65.50

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1256
variable_sites: 17
pi: 0.0101
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	CCCTTGAACTCCGCAATCTT	TTCCCTAGATACGCACATCG	50.0	50.0	58.2	56.9	1376-1382 bp	1.0	71.3
petD_p2	TTCCCTTGAACTCCGCAATC	TTCCCTAGATACGCACATCG	50.0	50.0	58.2	56.9	1378-1384 bp	1.0	71.3
petD_p3	CCCTTGAACTCCGCAATCTT	GCGACTATTCCCTAGATACGC	50.0	52.4	58.2	57.9	1383-1389 bp	1.0	70.9
petD_p4	TTCCCTTGAACTCCGCAATC	GCGACTATTCCCTAGATACGC	50.0	52.4	58.2	57.9	1385-1391 bp	1.0	70.8
petD_p5	TTTCCCTTGAACTCCGCAAT	TTCCCTAGATACGCACATCG	45.0	50.0	57.7	56.9	1379-1385 bp	1.0	70.4