Hotspot

trnN-GUU-trnR-ACG

IRa · 133769-134511 · 743 bp

Back to Vesalea

Pi 0.0198

MarkerSeek score 74.80

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 743
variable_sites: 21
pi: 0.0198
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	AGAAGTTCCGGGAGTTACGA	CCTTGTTCCTACATGACCCG	50.0	55.0	58.1	58.0	1604-1606 bp	1.0	84.4
trnN-GUU-trnR-ACG_p2	ATACGATAGCGGCTAGTCGA	CCTTGTTCCTACATGACCCG	50.0	55.0	57.9	58.0	2009-2022 bp	1.0	84.4
trnN-GUU-trnR-ACG_p3	AGAAGTTCCGGGAGTTACGA	CCTCGCAATCACTAGCCAAT	50.0	50.0	58.1	58.0	1437-1439 bp	1.0	84.4
trnN-GUU-trnR-ACG_p4	TGGGTTGAGAACGGGAATTG	CCTTGTTCCTACATGACCCG	50.0	55.0	58.1	58.0	1558-1560 bp	1.0	84.3
trnN-GUU-trnR-ACG_p5	AGAAGTTCCGGGAGTTACGA	TGTCCCCTCCAGTCAAGAAT	50.0	50.0	58.1	58.0	1462-1464 bp	1.0	84.3