Hotspot

trnN-GUU-ndhF

SSC · 114031-115067 · 1037 bp

Back to Vesalea

Pi 0.0187

MarkerSeek score 64.40

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: SSC
length_bp: 1037
variable_sites: 29
pi: 0.0187
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AGGATTCGAACCTACGACCA	GCTTTTTGTTGCTGTCGGAA	50.0	45.0	58.2	57.8	1204-1218 bp	1.0	79.4
trnN-GUU-ndhF_p2	AGGATTCGAACCTACGACCA	AAGGCTTTTTGTTGCTGTCG	50.0	45.0	58.2	57.8	1207-1221 bp	1.0	79.3
trnN-GUU-ndhF_p3	AGGATTCGAACCTACGACCA	CGAAGGCTTTTTGTTGCTGT	50.0	45.0	58.2	57.8	1209-1223 bp	1.0	79.3
trnN-GUU-ndhF_p4	GGATTCGAACCTACGACCAG	GCTTTTTGTTGCTGTCGGAA	55.0	45.0	57.8	57.8	1203-1217 bp	1.0	79.0
trnN-GUU-ndhF_p5	GGATTCGAACCTACGACCAG	AAGGCTTTTTGTTGCTGTCG	55.0	45.0	57.8	57.8	1206-1220 bp	1.0	78.9