Hotspot

rpl14-rpl16

LSC · 85276-85490 · 215 bp

Back to Vesalea

Pi 0.0156

MarkerSeek score 68.00

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 215
variable_sites: 5
pi: 0.0156
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	AGCATATCGGCGATTGCTAG	AGCAGCATCCAAAATGCCTA	50.0	45.0	58.0	57.9	352-363 bp	1.0	82.4
rpl14-rpl16_p2	AGCATATCGGCGATTGCTAG	TAGCAGCATCCAAAATGCCT	50.0	45.0	58.0	57.9	353-364 bp	1.0	82.4
rpl14-rpl16_p3	CATATCGGCGATTGCTAGCT	AGCAGCATCCAAAATGCCTA	50.0	45.0	58.0	57.9	350-361 bp	1.0	82.4
rpl14-rpl16_p4	CATATCGGCGATTGCTAGCT	TAGCAGCATCCAAAATGCCT	50.0	45.0	58.0	57.9	351-362 bp	1.0	82.4
rpl14-rpl16_p5	GAGCATATCGGCGATTGCTA	AGCAGCATCCAAAATGCCTA	50.0	45.0	58.0	57.9	353-364 bp	1.0	82.3