Hotspot

accD-psaI

LSC · 63141-63733 · 593 bp

Back to Vesalea

Pi 0.0438

MarkerSeek score 92.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 593
variable_sites: 39
pi: 0.0438
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 92.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCAATCTAGTCGTACCACGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	55.7	58.0	747-767 bp	1.0	69.9
accD-psaI_p2	TCAATCTAGTCGTACCACGT	AAGAAGCCATTGCAATTGCC	45.0	45.0	55.7	57.9	753-773 bp	1.0	69.2
accD-psaI_p3	TCAGTTCCATGGTTTCTTTCCT	CCATTGCAATTGCCGGAAAT	40.9	45.0	57.5	58.0	695-715 bp	1.0	68.6
accD-psaI_p4	TTCAATCTAGTCGTACCACGT	CCATTGCAATTGCCGGAAAT	42.9	45.0	56.5	58.0	748-768 bp	1.0	68.1
accD-psaI_p5	TCAGTTCCATGGTTTCTTTCCT	AAGAAGCCATTGCAATTGCC	40.9	45.0	57.5	57.9	701-721 bp	1.0	67.9