Hotspot

atpH-atpI

LSC · 14741-15921 · 1181 bp

Back to Vesalea

Pi 0.0113

MarkerSeek score 66.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1181
variable_sites: 17
pi: 0.0113
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	1269-1294 bp	1.0	77.1
atpH-atpI_p2	AGCCAATCCAGCAGCAATAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	57.9	58.5	1285-1310 bp	1.0	76.2
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.2	58.5	1270-1295 bp	1.0	75.9
atpH-atpI_p4	GCCAATCCAGCAGCAATAAC	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.8	58.5	1284-1309 bp	1.0	75.7
atpH-atpI_p5	ATAACGGAAGCGGCAGAAAT	CCGCGGCTTATATAGGTGAA	45.0	50.0	58.0	57.3	1254-1279 bp	1.0	75.4