Hotspot

ycf80

Genome · 54127-55557 · 1431 bp

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Pi 0.211
MarkerSeek score 80.00
Primer available yes
Label ycf80

Feature Metadata

label_name
ycf80
feature_type
gene
region
Genome
length_bp
1431
variable_sites
431
pi
0.211
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf80_p1 GGGTTCAAATCCCACCAGTT GCAGGAGAACATGGTCAACA 50.0 50.0 58.0 58.1 2162-2173 bp 0.666667 74.2
ycf80_p2 GGGTTCAAATCCCACCAGTT GAACATGGTCAACAAGTGCG 50.0 50.0 58.0 58.0 2167 bp 0.333333 53.0
ycf80_p3 GGGTTCAAATCCCACCAGTT TAGGTCTAGCGCCCACAATA 50.0 50.0 58.0 57.9 1971 bp 0.333333 52.6
ycf80_p4 AAACATGGACGTGGGTTCAA GAACATGGTCAACAAGTGCG 45.0 50.0 57.9 58.0 2179 bp 0.333333 52.6
ycf80_p5 AACATGGACGTGGGTTCAAA GAACATGGTCAACAAGTGCG 45.0 50.0 57.9 58.0 2178 bp 0.333333 52.6