Hotspot

ycf22

Genome · 46637-47284 · 648 bp

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Pi 0.188

MarkerSeek score 82.20

Primer available yes

Label ycf22

Feature Metadata

label_name: ycf22
feature_type: gene
region: Genome
length_bp: 648
variable_sites: 174
pi: 0.188
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf22_p1	ATAGGCATAGGCTCTGCTGT	CCGATTCCTAAAGCTCCACC	50.0	55.0	58.3	58.0	1582 bp	0.333333	50.8
ycf22_p2	ATAGGCATAGGCTCTGCTGT	GAATCTCCTACGCCGATTCC	50.0	55.0	58.3	57.9	1594 bp	0.333333	50.6
ycf22_p3	ATAGGCATAGGCTCTGCTGT	CTTTACGATATCCGCCAGGG	50.0	55.0	58.3	57.9	1374 bp	0.333333	50.5
ycf22_p4	ATAGGCATAGGCTCTGCTGT	CCTAAAGCTCCACCAGAACC	50.0	55.0	58.3	57.9	1576 bp	0.333333	50.2
ycf22_p5	ATAGGCATAGGCTCTGCTGT	ACCAGAACCTCCTTCTCCAA	50.0	50.0	58.3	57.9	1565 bp	0.333333	50.2