Hotspot

trpA

Genome · 33167-33979 · 813 bp

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Pi 0.189

MarkerSeek score 79.20

Primer available yes

Label trpA

Feature Metadata

label_name: trpA
feature_type: gene
region: Genome
length_bp: 813
variable_sites: 217
pi: 0.189
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trpA_p1	CGATTAGTTCCGGGTTCGAG	ATAAAAAGCAAGTGCCCCCA	55.0	45.0	58.2	58.0	1065 bp	0.333333	51.8
trpA_p2	CGATTAGTTCCGGGTTCGAG	TAAAAAGCAAGTGCCCCCAT	55.0	45.0	58.2	58.0	1064 bp	0.333333	51.8
trpA_p3	ACCTCCAGAAAGAGCGACTA	ATAAAAAGCAAGTGCCCCCA	50.0	45.0	57.8	58.0	1255 bp	0.333333	51.4
trpA_p4	ACCTCCAGAAAGAGCGACTA	TAAAAAGCAAGTGCCCCCAT	50.0	45.0	57.8	58.0	1254 bp	0.333333	51.4
trpA_p5	CGATTAGTTCCGGGTTCGAG	AGAAGCTATGGACAGCAGGA	55.0	50.0	58.2	58.1	1251 bp	0.333333	51.0