Hotspot

syfB

Genome · 155759-157726 · 1968 bp

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Pi 0.248

MarkerSeek score 78.60

Primer available yes

Label syfB

Feature Metadata

label_name: syfB
feature_type: gene
region: Genome
length_bp: 1968
variable_sites: 685
pi: 0.248
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
syfB_p1	TGCCACAGAAATATACCGCC	CGAATCCTCCTACCCCAGAT	50.0	55.0	58.0	58.0	2697-2743 bp	1.0	88.7
syfB_p2	GGTAAGGGTGCAATGGTTCA	CGAATCCTCCTACCCCAGAT	50.0	55.0	58.1	58.0	2667-2713 bp	1.0	88.4
syfB_p3	AGGAAAGTCCGGGCTCTATT	CGAATCCTCCTACCCCAGAT	50.0	55.0	58.1	58.0	2766-2812 bp	1.0	88.0
syfB_p4	TGCCACAGAAATATACCGCC	GGGTGTAGCCAAGTGGTAAG	50.0	55.0	58.0	57.9	2749-2795 bp	1.0	87.9
syfB_p5	TGCCACAGAAATATACCGCC	GGTGTAGCCAAGTGGTAAGG	50.0	55.0	58.0	57.9	2748-2794 bp	1.0	87.9