Hotspot

ompR

Genome · 166321-167046 · 726 bp

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Pi 0.18
MarkerSeek score 80.50
Primer available yes
Label ompR

Feature Metadata

label_name
ompR
feature_type
gene
region
Genome
length_bp
726
variable_sites
183
pi
0.18
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ompR_p1 CCACCTATCTGACACCAACG ACACAATTTTGTCAAGCCCT 55.0 40.0 58.0 56.0 1511 bp 0.333333 37.5
ompR_p2 AGCCGTGCGATTTCAAATTG ACACAATTTTGTCAAGCCCT 45.0 40.0 58.0 56.0 1589 bp 0.333333 37.5
ompR_p3 TAGGCAAAAGCCGATCAGTC ACACAATTTTGTCAAGCCCT 50.0 40.0 58.0 56.0 1566 bp 0.333333 37.4
ompR_p4 CCAACCGCCTAGTGCTAAAT ACACAATTTTGTCAAGCCCT 50.0 40.0 58.0 56.0 1329 bp 0.333333 37.3
ompR_p5 ACCCACCTATCTGACACCAA ACACAATTTTGTCAAGCCCT 50.0 40.0 58.0 56.0 1513 bp 0.333333 37.3