Hotspot

infB

Genome · 88287-90557 · 2271 bp

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Pi 0.202

MarkerSeek score 77.90

Primer available yes

Label infB

Feature Metadata

label_name: infB
feature_type: gene
region: Genome
length_bp: 2271
variable_sites: 648
pi: 0.202
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infB_p1	GTCACGTCGTTCTACTGGAC	TGTTTCCAGTACTGAGCAGC	55.0	50.0	58.0	57.8	2936 bp	0.333333	51.7
infB_p2	GTCACGTCGTTCTACTGGAC	TCCAGTACTGAGCAGCCTTA	55.0	50.0	58.0	57.8	2932 bp	0.333333	51.2
infB_p3	TCACGTCGTTCTACTGGACT	TGTTTCCAGTACTGAGCAGC	50.0	50.0	58.1	57.8	2935 bp	0.333333	51.1
infB_p4	GTCACGTCGTTCTACTGGAC	TTCACCTTCTGACTGTGCAG	55.0	50.0	58.0	57.8	2652 bp	0.333333	51.1
infB_p5	TCACGTCGTTCTACTGGACT	TCCAGTACTGAGCAGCCTTA	50.0	50.0	58.1	57.8	2931 bp	0.333333	50.6