Hotspot

trnL-trnN

Genome · 22738-22851 · 114 bp

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Pi 0.313

MarkerSeek score 83.70

Primer available yes

Label trnL-trnN

Feature Metadata

label_name: trnL-trnN
feature_type: igs
region: Genome
length_bp: 114
variable_sites: 48
pi: 0.313
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-trnN_p1	TAGCGTGTCTGCCAATTTCA	ACTCCCCAGGAAGGATTTGA	45.0	50.0	57.8	57.9	217-219 bp	1.0	88.7
trnL-trnN_p2	TAGCGTGTCTGCCAATTTCA	CCCCAGGAAGGATTTGAACC	45.0	55.0	57.8	58.1	214-216 bp	1.0	87.6
trnL-trnN_p3	TAGCGTGTCTGCCAATTTCA	ACCACTGAGCTACTGAGGAA	45.0	50.0	57.8	57.7	163-165 bp	1.0	87.0
trnL-trnN_p4	GTGTCTGCCAATTTCACCAC	ACTCCCCAGGAAGGATTTGA	50.0	50.0	57.6	57.9	213-215 bp	1.0	86.8
trnL-trnN_p5	TCTAGCGTGTCTGCCAATTT	ACTCCCCAGGAAGGATTTGA	45.0	50.0	57.5	57.9	219-221 bp	1.0	86.3