Hotspot

ConsOrf4

Genome · 68922-69653 · 732 bp

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Pi 0.27

MarkerSeek score 78.80

Primer available yes

Label ConsOrf4

Feature Metadata

label_name: ConsOrf4
feature_type: gene
region: Genome
length_bp: 732
variable_sites: 278
pi: 0.27
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ConsOrf4_p1	CGGGTCTTCCTGTTGAACAA	GAACCGTCGCTAAATGTTGC	50.0	50.0	58.0	58.1	1860 bp	0.333333	52.1
ConsOrf4_p2	GCGTTTACCAAATTTCGCCA	GAACCGTCGCTAAATGTTGC	45.0	50.0	57.9	58.1	1488 bp	0.333333	51.9
ConsOrf4_p3	TGCGTTTACCAAATTTCGCC	GAACCGTCGCTAAATGTTGC	45.0	50.0	57.9	58.1	1489 bp	0.333333	51.9
ConsOrf4_p4	CGGGTCTTCCTGTTGAACAA	TCTTAGCGCTCAAGCCTTTT	50.0	45.0	58.0	57.8	1394 bp	0.333333	51.5
ConsOrf4_p5	GCGTTTACCAAATTTCGCCA	TCTTAGCGCTCAAGCCTTTT	45.0	45.0	57.9	57.8	1022 bp	0.333333	51.4