Hotspot

ycf1

SSC · 122824-128250 · 5427 bp

Back to Veronicastrum

Pi 0.00972

MarkerSeek score 52.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5427
variable_sites: 195
pi: 0.00972
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GATTGTTGTGAATAGCGTAG	CCGAGTTAGAACAACAATCAGG	40.0	45.5	52.2	57.1	172-187 bp	1.0	51.1
ycf1_p2	GATTGTTGTGAATAGCGTAG	CACCGAGTTAGAACAACAATCAG	40.0	43.5	52.2	57.7	174-189 bp	1.0	51.0
ycf1_p3	GATTGTTGTGAATAGCGTAG	CACCGAGTTAGAACAACAATCA	40.0	40.9	52.2	56.7	174-189 bp	1.0	51.0
ycf1_p4	GATTGTTGTGAATAGCGTAG	TCACCGAGTTAGAACAACAATCA	40.0	39.1	52.2	58.0	175-190 bp	1.0	50.9
ycf1_p5	GATTGTTGTGAATAGCGTAG	TCACCGAGTTAGAACAACAA	40.0	40.0	52.2	54.6	175-190 bp	1.0	50.9