Hotspot

ndhD-psaC

SSC · 116134-116234 · 101 bp

Back to Veronicastrum

Pi 0.0548

MarkerSeek score 71.70

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 101
variable_sites: 13
pi: 0.0548
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	GAACCCGCAAATATTGGCAA	AAGAGATGTGAATCCGCCTG	45.0	50.0	57.3	57.7	277 bp	1.0	76.4
ndhD-psaC_p2	GAACCCGCAAATATTGGCAA	GAGATGTGAATCCGCCTGTC	45.0	55.0	57.3	58.4	275 bp	1.0	75.7
ndhD-psaC_p3	GAACCCGCAAATATTGGCAA	GCCTGTCCAACGGATTTCTT	45.0	50.0	57.3	58.5	262 bp	1.0	75.5
ndhD-psaC_p4	GCCCCTATGTGGAAGAAGGA	AAGAGATGTGAATCCGCCTG	55.0	50.0	58.8	57.7	305 bp	1.0	75.1
ndhD-psaC_p5	TTGCCCCTATGTGGAAGAAG	AAGAGATGTGAATCCGCCTG	50.0	50.0	57.2	57.7	307 bp	1.0	74.9