Hotspot

rpl32-trnL-UAG

SSC · 112424-113162 · 739 bp

Back to Veronicastrum

Pi 0.013
MarkerSeek score 62.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
739
variable_sites
30
pi
0.013
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TTCCCCGTTCTCGAGGTATT AGCGTGTCTACCGATTTCAC 50.0 50.0 58.1 58.0 1351-1375 bp 0.6 69.0
rpl32-trnL-UAG_p2 TTTCCCCGTTCTCGAGGTAT AGCGTGTCTACCGATTTCAC 50.0 50.0 58.1 58.0 1352-1376 bp 0.6 69.0
rpl32-trnL-UAG_p3 TTCCCCGTTCTCGAGGTATT CAGCGTGTCTACCGATTTCA 50.0 50.0 58.1 58.0 1352-1376 bp 0.6 69.0
rpl32-trnL-UAG_p4 TTTCCCCGTTCTCGAGGTAT CAGCGTGTCTACCGATTTCA 50.0 50.0 58.1 58.0 1353-1377 bp 0.6 69.0
rpl32-trnL-UAG_p5 TTCCCCGTTCTCGAGGTATT AAAAATAGCACTGGGCCCAA 50.0 45.0 58.1 58.0 1878-1924 bp 0.6 68.9