Hotspot

ndhF-rpl32

SSC · 111529-112258 · 730 bp

Back to Veronicastrum

Pi 0.0484

MarkerSeek score 81.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 730
variable_sites: 80
pi: 0.0484
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACGAAAGGTATGATCCACGC	AGAAGGTTGCACAAAAGGGT	50.0	45.0	58.1	57.8	1201-1403 bp	1.0	87.3
ndhF-rpl32_p2	GCCCACATACGACGAAGATT	AGAAGGTTGCACAAAAGGGT	50.0	45.0	58.1	57.8	1282-1484 bp	1.0	87.0
ndhF-rpl32_p3	ACGAAAGGTATGATCCACGC	CTCCCCACCTTGGAAATGAG	50.0	55.0	58.1	57.9	1020-1222 bp	0.7	73.8
ndhF-rpl32_p4	CGAAAGGTATGATCCACGCA	CTCCCCACCTTGGAAATGAG	50.0	55.0	58.1	57.9	1019-1221 bp	0.7	73.8
ndhF-rpl32_p5	GCCCACATACGACGAAGATT	CTCCCCACCTTGGAAATGAG	50.0	55.0	58.1	57.9	1101-1303 bp	0.7	73.4