Hotspot

psbH-petB

LSC · 74340-74475 · 136 bp

Back to Veronicastrum

Pi 0.0132

MarkerSeek score 63.70

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 136
variable_sites: 7
pi: 0.0132
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	GTCGCAATGGCTCTATTTGC	TCTATCAAGGTGACAGGCCT	50.0	50.0	57.9	57.7	281-294 bp	1.0	83.0
psbH-petB_p2	AACTCCTTTGATGGGTGTCG	TCTATCAAGGTGACAGGCCT	50.0	50.0	57.8	57.7	297-310 bp	1.0	82.3
psbH-petB_p3	CTTTGATGGGTGTCGCAATG	TCTATCAAGGTGACAGGCCT	50.0	50.0	57.7	57.7	292-305 bp	1.0	81.7
psbH-petB_p4	GGTGTCGCAATGGCTCTATT	TCTATCAAGGTGACAGGCCT	50.0	50.0	58.3	57.7	284-297 bp	1.0	81.2
psbH-petB_p5	GTGGGGAACAACTCCTTTGA	TCTATCAAGGTGACAGGCCT	50.0	50.0	57.6	57.7	306-319 bp	1.0	80.9