Hotspot

ndhC-trnV-UAC

LSC · 50234-51342 · 1109 bp

Back to Veronicastrum

Pi 0.0125
MarkerSeek score 62.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1109
variable_sites
40
pi
0.0125
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TTCTCCGGCCCTTTCCTAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.4 57.8 1256-1312 bp 1.0 78.5
ndhC-trnV-UAC_p2 TCTCCGGCCCTTTCCTAATA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.5 57.8 1255-1311 bp 1.0 77.9
ndhC-trnV-UAC_p3 TCCGGCCCTTTCCTAATAGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.6 57.8 1253-1309 bp 1.0 77.3
ndhC-trnV-UAC_p4 TTTCTCCGGCCCTTTCCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.6 57.8 1257-1313 bp 1.0 76.7
ndhC-trnV-UAC_p5 TTCTCCGGCCCTTTCCTAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 58.4 57.5 1255-1311 bp 1.0 75.8