Hotspot

trnK-UUU-rps16

LSC · 4247-5111 · 865 bp

Back to Veronicastrum

Pi 0.0103

MarkerSeek score 65.40

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 865
variable_sites: 30
pi: 0.0103
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	654-1018 bp	1.0	85.0
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	653-1017 bp	1.0	85.0
trnK-UUU-rps16_p3	TCTAGCCGCACTTAAAAGCC	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.3	58.0	668-1032 bp	1.0	84.2
trnK-UUU-rps16_p4	CCGCACTTAAAAGCCGAGTA	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.3	58.0	663-1027 bp	1.0	84.1
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	CTTGAAAAAGGCGCTCAACC	50.0	50.0	58.2	58.2	657-1021 bp	1.0	83.4