Hotspot

trnH-GUG-psbA

LSC · 86-409 · 324 bp

Back to Veronicastrum

Pi 0.0337

MarkerSeek score 83.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 324
variable_sites: 38
pi: 0.0337
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	351-394 bp	1.0	90.4
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	365-408 bp	1.0	90.3
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	364-407 bp	1.0	90.3
trnH-GUG-psbA_p4	ATTAGTCATGGGCGAACGAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.1	57.9	414-457 bp	0.8	80.6
trnH-GUG-psbA_p5	CATTAGTCATGGGCGAACGA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.1	57.9	415-458 bp	0.8	80.5