Hotspot

ycf1

SSC · 120723-125987 · 5265 bp

Back to Veronica

Pi 0.0531

MarkerSeek score 75.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5265
variable_sites: 1080
pi: 0.0531
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCTTCCCTTTTCTCCAACC	TGACAGAGCGGTCAGAAATG	50.0	50.0	58.0	57.9	1120-1150 bp	0.32	61.4
ycf1_p2	TGCTTCCCTTTTCTCCAACC	GCAGAATACCGTCACCCATT	50.0	50.0	58.0	58.0	1463-1493 bp	0.32	60.3
ycf1_p3	TGCTTCCCTTTTCTCCAACC	ATCACTTTGACAGAGCGGTC	50.0	50.0	58.0	57.9	1127-1157 bp	0.24	57.6
ycf1_p4	TGCTTCCCTTTTCTCCAACC	AAAAATGGACCCGTTTCCGA	50.0	45.0	58.0	58.0	1329 bp	0.12	44.6
ycf1_p5	TGCTTCCCTTTTCTCCAACC	AAAATGGACCCGTTTCCGAA	50.0	45.0	58.0	58.0	1328 bp	0.12	44.6