Hotspot

rps15-ycf1

SSC · 120337-120722 · 386 bp

Back to Veronica

Pi 0.103

MarkerSeek score 70.60

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 386
variable_sites: 127
pi: 0.103
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CGTTGGCGTTTTCCCAAAAT	AGCACCAAAATAGCGTCGAT	45.0	45.0	58.1	58.0	825-935 bp	0.32	63.5
rps15-ycf1_p2	GGTCTGTGCCCCAATATCTG	AGCACCAAAATAGCGTCGAT	55.0	45.0	58.0	58.0	1386-1499 bp	0.32	63.1
rps15-ycf1_p3	GGTCTGTGCCCCAATATCTG	AAAACAGCAGGCAAGCTTTG	55.0	45.0	58.0	58.1	1332-1350 bp	0.24	58.8
rps15-ycf1_p4	AGCTGTTCGGGTCCATTTAC	AGCACCAAAATAGCGTCGAT	50.0	45.0	57.9	58.0	1265-1283 bp	0.24	58.8
rps15-ycf1_p5	AGCTGTTCGGGTCCATTTAC	AAAACAGCAGGCAAGCTTTG	50.0	45.0	57.9	58.1	1211-1229 bp	0.24	58.5