Hotspot

rpl32-trnL-UAG

SSC · 110331-111088 · 758 bp

Back to Veronica

Pi 0.0815

MarkerSeek score 74.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 758
variable_sites: 218
pi: 0.0815
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CGGCGTTAAAAGCGTTTTCT	CACACAAAAGTGCCGCATAG	45.0	50.0	58.0	58.0	1492-1661 bp	0.64	77.1
rpl32-trnL-UAG_p2	CGGCGTTAAAAGCGTTTTCT	AACCACTCCGATTGAAGAGC	45.0	50.0	58.0	57.9	1442-1611 bp	0.64	76.6
rpl32-trnL-UAG_p3	CGGCGTTAAAAGCGTTTTCT	AAAAGTGCCGCATAGCCTAA	45.0	45.0	58.0	57.9	1487-1656 bp	0.64	76.4
rpl32-trnL-UAG_p4	CGGCGTTAAAAGCGTTTTCT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.0	57.9	880-995 bp	0.64	76.4
rpl32-trnL-UAG_p5	AAAGAAAGGGGGATCAAGCG	CACACAAAAGTGCCGCATAG	50.0	50.0	58.2	58.0	1510-1530 bp	0.4	66.5