Hotspot

petA-psbJ

LSC · 61746-62680 · 935 bp

Back to Veronica

Pi 0.0561

MarkerSeek score 76.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 935
variable_sites: 205
pi: 0.0561
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TAGGCGGTAATAGGGGAAGG	ACACAATCAAACCCGAACGA	55.0	45.0	58.0	58.0	1644-1701 bp	1.0	91.5
petA-psbJ_p2	TGTTGGTGGATTTGGTCAGG	ACACAATCAAACCCGAACGA	50.0	45.0	58.0	58.0	1397-1454 bp	0.92	88.4
petA-psbJ_p3	TGTTGGTGGATTTGGTCAGG	GTTCATGGCCTAGCTGTACC	50.0	55.0	58.0	58.0	1495-1552 bp	0.92	88.4
petA-psbJ_p4	TGTTGGTGGATTTGGTCAGG	TCCCTATTCATTGCGGGTTG	50.0	50.0	58.0	57.9	1725-1782 bp	0.92	88.3
petA-psbJ_p5	TGTTGGTGGATTTGGTCAGG	GAGCCGACAAGGAATTCCAT	50.0	50.0	58.0	58.0	1636-1693 bp	0.88	86.8