Hotspot

trnT-UGU-trnL-UAA

LSC · 45510-46228 · 719 bp

Back to Veronica

Pi 0.0804

MarkerSeek score 77.90

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 719
variable_sites: 198
pi: 0.0804
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	646-814 bp	1.0	89.0
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	640-808 bp	1.0	87.7
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CGTAGCGTCTACCGATTTCG	55.0	55.0	57.9	58.6	645-813 bp	1.0	86.0
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	TCCGTAGCGTCTACCGATTT	55.0	50.0	57.9	58.6	647-815 bp	1.0	86.0
trnT-UGU-trnL-UAA_p5	ATGCGATGCTCTAACCTCTG	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.5	58.2	648-816 bp	1.0	85.8