Hotspot

trnT-GGU-psbD

LSC · 30374-31690 · 1317 bp

Back to Veronica

Pi 0.053

MarkerSeek score 82.10

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1317
variable_sites: 263
pi: 0.053
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGCGTAAGTCATCGGTTCAA	TTTACCAAGGGCTATAGTCA	50.0	40.0	58.3	53.0	1333-1425 bp	1.0	52.0
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	TTTACCAAGGGCTATAGTCA	50.0	40.0	57.9	53.0	1409-1462 bp	0.96	50.4
trnT-GGU-psbD_p3	GCCCTTTTAACTCAGCGGTA	TTACCAAGGGCTATAGTCAT	50.0	40.0	57.9	52.8	1408-1461 bp	0.96	50.4
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	ATTTACCAAGGGCTATAGTCA	50.0	38.1	57.9	53.7	1410-1463 bp	0.96	50.4
trnT-GGU-psbD_p5	GCCCTTTTAACTCAGCGGTA	TTTACCAAGGGCTATAGTCAT	50.0	38.1	57.9	53.7	1409-1462 bp	0.96	50.4