Hotspot

trnE-UUC-trnT-GGU

LSC · 29839-30301 · 463 bp

Back to Veronica

Pi 0.0512

MarkerSeek score 77.10

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 463
variable_sites: 93
pi: 0.0512
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AAACCACTAGACGATGGGGG	GAACCGATGACTTACGCCTT	55.0	50.0	59.1	58.0	539-636 bp	1.0	83.2
trnE-UUC-trnT-GGU_p2	AAACCACTAGACGATGGGGG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	59.1	57.9	504-601 bp	1.0	82.4
trnE-UUC-trnT-GGU_p3	AAACCACTAGACGATGGGGG	CCGATGACTTACGCCTTACC	55.0	55.0	59.1	58.2	536-633 bp	1.0	82.1
trnE-UUC-trnT-GGU_p4	AAACCACTAGACGATGGGGG	TTGAACCGATGACTTACGCC	55.0	50.0	59.1	58.3	541-638 bp	1.0	81.0
trnE-UUC-trnT-GGU_p5	AACCACTAGACGATGGGGG	GAACCGATGACTTACGCCTT	57.9	50.0	58.4	58.0	538-635 bp	1.0	80.8