Hotspot

rpoB-trnC-GCA

LSC · 26098-27328 · 1231 bp

Back to Veronica

Pi 0.0549

MarkerSeek score 76.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1231
variable_sites: 239
pi: 0.0549
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCGCCTACACAAGCAAATTG	CGCTGCTTCTCACAGGTAAT	50.0	50.0	58.0	58.0	2008-2088 bp	0.92	88.5
rpoB-trnC-GCA_p2	CGAGCCCATATCCTTGCTTT	CGCTGCTTCTCACAGGTAAT	50.0	50.0	58.0	58.0	1816-1896 bp	0.92	88.5
rpoB-trnC-GCA_p3	CGAGCCCATATCCTTGCTTT	ACCCTCATAGATTCTCGCGA	50.0	50.0	58.0	58.0	2066-2124 bp	0.64	77.2
rpoB-trnC-GCA_p4	CCGCCTACACAAGCAAATTG	ACCCTCATAGATTCTCGCGA	50.0	50.0	58.0	58.0	2258-2316 bp	0.64	77.1
rpoB-trnC-GCA_p5	CCGCCTACACAAGCAAATTG	CATAGATTCTCGCGAGTCCG	50.0	55.0	58.0	58.0	2253-2311 bp	0.64	76.9