Hotspot

atpH-atpI

LSC · 13089-13874 · 786 bp

Back to Veronica

Pi 0.055

MarkerSeek score 77.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 786
variable_sites: 163
pi: 0.055
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TCCTCATCTGCAAATCAGCC	GGCAAATAGGGGGTTTCCAA	50.0	50.0	58.0	58.1	1801-2056 bp	0.96	89.6
atpH-atpI_p2	TGGCCCAAAGAAACGAAAGA	TTTCCAAGTCCACGGACAAG	45.0	50.0	57.9	58.0	2072-2284 bp	0.4	63.6
atpH-atpI_p3	TCCTCATCTGCAAATCAGCC	TTTCCAAGTCCACGGACAAG	50.0	50.0	58.0	58.0	1819-2034 bp	0.4	63.5
atpH-atpI_p4	CTCATCTGCAAATCAGCCCT	TTTCCAAGTCCACGGACAAG	50.0	50.0	57.9	58.0	1817-2032 bp	0.4	63.5
atpH-atpI_p5	CACACACACTCCCTTTCCAA	TTTCCAAGTCCACGGACAAG	50.0	50.0	57.9	58.0	2184-2396 bp	0.4	63.4